Dehydrogenase/Carbonyl Reductase Gene in Comamonas testosteroni
نویسندگان
چکیده
Wenjie Gong, Guangming Xiong, Edmund Maser* 5 6 Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School, Schleswig7 Holstein, Campus Kiel, Brunswiker Strasse 10,D-24105 Kiel, Germany 8 9 *Corresponding author: Prof. Edmund Maser, Tel.: +49-431-597-3540; fax: +49-431-597-3558; 10 E-mail address: [email protected] (E. Maser) 11 12 Running Title: HsdR positively regultates the 3α-HSD/CR expression. 13 14 15 16 17 18 19 20 21 22 23 24 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.06872-11 AEM Accepts, published online ahead of print on 9 December 2011
منابع مشابه
Molecular Cloning, Overexpression, and Characterization of Steroid-inducible 3a-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosteroni A NOVEL MEMBER OF THE SHORT-CHAIN DEHYDROGENASE/REDUCTASE SUPERFAMILY*
3a-Hydroxysteroid dehydrogenase/carbonyl reductase (3a-HSD/CR) from Comamonas testosteroni, a bacterium that is able to grow on steroids as the sole carbon source, catalyzes the oxidoreduction at position 3 of a variety of C19–27 steroids and the carbonyl reduction of a variety of nonsteroidal aldehydes and ketones. The gene of this steroid-inducible 3a-HSD/CR was cloned by screening a C. testo...
متن کاملMolecular Cloning, Overexpression, and Characterization of Steroid-inducible 3a-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosteroni
3a-Hydroxysteroid dehydrogenase/carbonyl reductase (3a-HSD/CR) from Comamonas testosteroni, a bacterium that is able to grow on steroids as the sole carbon source, catalyzes the oxidoreduction at position 3 of a variety of C19–27 steroids and the carbonyl reduction of a variety of nonsteroidal aldehydes and ketones. The gene of this steroid-inducible 3a-HSD/CR was cloned by screening a C. testo...
متن کاملThe Catalytic Roles of P185 and T188 and Substrate-Binding Loop Flexibility in 3α-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosteroni
3α-Hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni reversibly catalyzes the oxidation of androsterone with NAD(+) to form androstanedione and NADH. Structurally the substrate-binding loop of the residues, T188-K208, is unresolved, while binding with NAD(+) causes the appearance of T188-P191 in the binary complex. This study determines the functional roles of the flex...
متن کاملA new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes.
In Comamonas testosteroni TA441, testosterone is degraded via aromatization of the A ring, which is cleaved by the meta-cleavage enzyme TesB, and further degraded by TesD, the hydrolase for the product of TesB. TesEFG, encoded downstream of TesD, are probably hydratase, aldolase, and dehydrogenase for degradation of 2-oxohex-4-enoicacid, one of the products of TesD. Here we present a new and un...
متن کاملMolecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D.
Comamonas testosteroni YZW-D was isolated from Passaic River sediment for its ability to degrade isophthalate and terephthalate. Degradation of the two isomeric compounds proceeds via separately inducible catabolic pathways that converge at protocatechuate. Analysis of the catabolic pathways by which these two isomers are degraded demonstrated that a cis-dihydrodiol intermediate is involved in ...
متن کاملTestosterone-inducible regulator is a kinase that drives steroid sensing and metabolism in Comamonas testosteroni.
The mechanism of gene regulation by steroids in bacteria is still a mystery. We use steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) as a reporter system to study steroid signaling in Comamonas testosteroni. In previous investigations we cloned and characterized the 3alpha-HSD/CR-encoding gene, hsdA. In addition, we identified two negative regulator genes...
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تاریخ انتشار 2011